The court said alternative routes for cruise ships have not been determined.
In November, the government moved to ban cruise ships of more than 96,000 gross tons from the Giudecca Canal en route to the main cruise docks operated by Venezia Terminal Passeggeri (VTP). That limit was scheduled to take effect in November 2014Court .
The order also cut back the number of ships more than 40,000 gross tons using the route, effective January 2014.
An appeal was filed by VTP, several port businesses and a cruise line operators committee.
According to Italian newspaper Gazetta del Sud, a hearing to further consider the ban is scheduled for June 12.